▎ 摘　　要

In this study, we optimized the reforming of bilirubin oxidase (BOD) using graphene-coated carbon fiber woven fabric (GCFC) as an electrode, and The performance of the cathode in which synthesized 1-pyrene butyric acid N-hydroxysuccinimide ester (PBSE) and 2,5-dimethyl-1-phenyl-1-H-pyrrole-3-carbaldehyde (DPPC) were added, was evaluated. In addition, the prototype evaluation of the atmospheric-exposure-type ascorbic acid enzyme biofuel cell (AAEBFC) using the improved GCFC cathode was performed. The area of both the anode and cathode electrodes was 5mm x 5 mm. No modification was performed to the anode, and only the cathode was coated with the enzyme BOD. In the work, for the AAEBFC using the BOD-modified cathode, an output of 238.5 mu W/cm(2) was obtained at 0.245 V. Further, in the AAEBFC using the DPPC-PBSE-BOD-modified cathode, an output of 338.8 mu W/cm(2) was obtained at 0.292 V. The output in this work was improved by approximately 1.4 times by the additives.