• 文献标题:   Functionalized graphene quantum dots loaded with free radicals combined with liquid chromatography and tandem mass spectrometry to screen radical scavenging natural antioxidants from Licorice and Scutellariae
  • 文献类型:   Article
  • 作  者:   WANG GY, NIU XL, SHI GF, CHEN XF, YAO RX, CHEN FW
  • 作者关键词:   antioxidant, free radical, graphene, quantum dot, radical scavenger
  • 出版物名称:   JOURNAL OF SEPARATION SCIENCE
  • ISSN:   1615-9306 EI 1615-9314
  • 通讯作者地址:   Lanzhou Univ Technol
  • 被引频次:   9
  • DOI:   10.1002/jssc.201400622
  • 出版年:   2014

▎ 摘  要

A novel screening method was developed for the detection and identification of radical scavenging natural antioxidants based on a free radical reaction combined with liquid chromatography with tandem mass spectrometry. Functionalized graphene quantum dots were prepared for loading free radicals in the complex screening system. The detection was performed with and without a preliminary exposure of the samples to specific free radicals on the functionalized graphene quantum dots, which can facilitate charge transfer between free radicals and antioxidants. The difference in chromatographic peak areas was used to identify potential antioxidants. This is a novel approach to simultaneously evaluate the antioxidant power of a component versus a free radical, and to identify it in a vegetal matrix. The structures of the antioxidants in the samples were identified using tandem mass spectrometry and comparison with standards. Fourteen compounds were found to possess potential antioxidant activity, and their free radical scavenging capacities were investigated. The order of scavenging capacity of 14 compounds was compared according to their free radical scavenging rate. 4',5,6,7-Tetrahydroxyflavone (radical scavenging rate: 0.05253 mL mg(-1) s(-1)) showed the strongest capability for scavenging free radicals.