▎ 摘　　要

A cost-effective mass production of pristine graphene nanosheet is still an important challenge to harness its maximum potential in medical sciences. Here, we have used a binary solvent mixture of low dielectric constant (1,2,4- trichlorobenzene,epsilon = 2.24 and benzylamine,epsilon = 4.6) to produce a high quantity (0.6 mg/ml) of graphene under ultrasonication without compromising its quality. Obtained sample were characterized through XRD, UV-Vis, Raman, FT-IR, TEM-SAED, and cytopotency was evaluated on SiHa cell line. I-G/I-2D < 1.0 (similar to 0.39) was achieved on more than 250 mu m(2) area, indicating the uniformity and obtained I-D/I-G ratio was 1.21. No toxicity was found in the case of 24 hr on the SiHa cell line. EC50 computation was 84.90 mu g /ml for 48 hr and 62.03 mu g /ml for 72 hr. Exfoliated graphene remained stable in binary solvent even after 1 year. The proposed experiment and obtained data can be powerful tools to explore the synergic effect of binary solvent exfoliation in combination to establish graphene as a biomaterial for neuronal network, tissue engineering, drug delivery, and regenerative medicines, to name a few.