▎ 摘　　要

Hybridizing nanometals and carbon nanosheets as a nanocomposite has resulted in the best-performing biosensors to date. The potential of an amperometric L-phenylalanine (L-phe) biosensor, based on the immobilization of phenylalanine dehydrogenase (PDH) onto a nanocomposite, containing a hybrid of reduced graphene nanosheets (rGNS) and silver nanoparticles (AgNPs) electrodeposited on glassy carbon electrodes, was investigated. The two-dimensional support (rGNS) was incorporated to zero-dimensional AgNPs arrayed in the third dimension and developed as an L-phe biosensor with excellent biosensing capabilities. Biosensor versatility was developed using a novel nanocomposite that permitted easy changes in sensor performance (i.e., sensitivity, limit of detection, and linear range). This method resulted in a high sensitive biosensor design that exhibits excellent sensing performance including improved L-phe sensitivity (47 nM limit of detection, 0.15-900 mu M linear sensing range), possesses a long and stable shelf life (> 1 month), and high selectivity.