▎ 摘　　要

A ratiometric electrochemical sensor was constructed for sensitive 4-acetamidophenol (4-AP) detection. Ferrocene (Fc) was attached onto a self-assembly of graphene oxide (GO) to form a GO-Fc complex with electrostatic interactions. Furthermore, Nafion was added into the complex in a certain ratio to immobilize the complex. The GO-Fc-Nafion complex was drop-coated on the surface of a glassy carbon electrode (GCE) to prepare a GO-Fc-Nafion/GCE sensing platform. There was a strong, plotted linear relationship between I4-AP/I-Fc and 4-AP concentration in the range from 1 to 100 mu M, with a low detection limit of 0.2 mu M. This fabricated ratiometric electrochemical sensing platform exhibited high sensitivity and stability responses towards 4-AP, over potential interfering species. Experimental results confirmed high detection stability and recoveries, indicating its high feasibility for 4-AP detection in biological samples.