▎ 摘　　要

Developing a noninvasive skin peroxide monitoring technologyishighly desirable for managing a number of metabolic disorders associatedwith diabetes, pulmonary diseases, or other health conditions. Todate, the majority of studies on peroxide detection have been conductedon simulated sweat and high pH conditions, which are beyond the physiologicalrange, in order to provide an enhanced response. Here, a skin-wornamperometric sensor, based on laser-induced graphene (LIG), surface-engineeredwith a Prussian blue (PB)-chitosan (CS) network was fabricatedby one-step electrodeposition for the stable and sensitive detectionof H2O2 in human eccrine perspiration. The hybrid(PB-CS) network and laser-written electrode configuration wereoptimized through systematic investigation of electrodeposition parametersand concurrent feedback from electrochemical and morphological characterizationat each fabrication step. Different from the multitude of carbon-basedelectrodes functionalized with metallic nanoparticles reported inthe literature, the sensor was operated at a low potential of -0.036V vs Ag/AgCl in unmodified human eccrine perspiration. The low workingpotential ensured that the sensor is highly specific, immune to thecurrent from oxygen reduction or common interfering species, whereasthe inclusion of CS in the hybrid coating afforded a highly stableperformance over a period of 14 days. The sensor was able to monitorH(2)O(2) over the linear range of 10-1000 mu M with a low detection limit of 6.31 mu M and achieveda recovery of 98.73% (%RSD 0.86) in human eccrine perspiration. Thisfacile biosensor based on directly laser-written electrodes coupledwith the one-step PB-CS fabrication strategy has the potentialto form the basis for the development of oxidase enzyme-based sensorsin sweat.