▎ 摘　　要

A graphene/beta-cyclodextrin biosensor with tyrosinase was developed by combining the enhanced sensitivity given by the special properties of graphene and cyclodextrin, and the specificity of the enzyme for dopamine detection. The nanoplatform was achieved by layer-by-layer deposition and various parameters were optimized by using square wave voltammetry. Microscopic and spectral techniques (Raman, FTIR) were used to characterize the modification of graphene oxide and of the glassy carbon electrodes. The biosensor was successfully applied for dopamine determination in pharmaceutical products, human serum and urine with good recoveries, by using amperometry.