• 文献标题:   A disposable immunosensor for Enterobacter sakazakii based on an electrochemically reduced graphene oxide-modified electrode
  • 文献类型:   Article
  • 作  者:   HU X, DOU WC, FU LL, ZHAO GY
  • 作者关键词:   electrochemically reduced graphene oxide, immunosensor, enterobacter sakazakii
  • 出版物名称:   ANALYTICAL BIOCHEMISTRY
  • ISSN:   0003-2697 EI 1096-0309
  • 通讯作者地址:   Zhejiang Gongshang Univ
  • 被引频次:   13
  • DOI:   10.1016/j.ab.2012.11.013
  • 出版年:   2013

▎ 摘  要

A disposable immunosensor based on electrochemically reduced graphene oxide (ERGO) was developed for the detection of Enterobacter sakazakii. First, the graphene was deposited on a screen-printed carbon electrode (SPCE) by an electrochemical method. Second, the horseradish peroxidase-labeled bacteria-specific antibody was assembled onto the modified electrode to enhance the sensitivity of the immunosensor. The immunosensor constructed in this study can sensitively and rapidly detect E. sakazakii, and this method may contribute to further foodborne pathogen detection. In addition, this ERGO-modified SPCE could also provide new insights into the development of immunosensors for other bacteria to facilitate rapid detection. (c) 2012 Elsevier Inc. All rights reserved.