▎ 摘　　要

Graphene oxide (GO) is extensively recognized as an effective material in a variety of fields and specifically for various biomedical applications. However, the modification of each side of a GO sheet, needed for preparation of heterogeneous nanoparticles, is yet hypothetical and unclear. Here, we have developed a new strategy for the synthesis of two-faced GOs by simultaneous anchoring of different polymers with different hydro-affinity via Pickering emulsion (two-phase media). Alkynyl-modified GO was prepared for unsymmetrically attaching azidated-poly(3-caprolactone) (PCL) and (N-isopropylacrylamide-co-acrylamide-co-allylamine) terpolymer to the surface of GO through click reaction. During this reaction, a thermoresponsive nanoparticles with specific morphology, known as "Janus" nanostructure, will be produced. PCL/terpolymer homostructure was also fabricated via solution (one-phase) reaction, where two types of polymers were grafted on the both surfaces of GO disorderly, named "mixed" nanoparticles. In the following, drug loaded nanocarriers were synthesized by simultaneously using quercetin and 5-FU as hydrophobic and hydrophilic drugs. The bioapplicability of these nanoparticles were evaluated by comparison of their in vitro release profiles and cell cytotoxicity at two temperatures of 37 and 40 degrees C. (C) 2018 Elsevier Ltd. All rights reserved.