▎ 摘　　要

Burn wound infection is highly serious and causes huge mortality and morbidity in hospitals. Various treatment options are available for the wound management but the majority of them produce numerous side effects. Hence, the present study is aimed to produce gabapentin loaded graphene-keratin (GP@GO-KR) nanocomposite and its antibacterial and burn wound healing properties were tested. The synthesized nanocomposite was characterized by biophysical techniques (UV, XRD, FTIR and SEM). A strong interaction between the three (gabapentin, graphene and keratin) molecules was confirmed by FTIR spectra. SEM analysis confirmed the nanosheets structures. The presence of crystalline material was confirmed by XRD. The newly formed GP@GO-KR nanocomposite showed higher antibacterial activity against S. aureus and E. coli. In addition, the confocal laser scanning microscopic (CLSM) studies showed that GP@GO-KR nanocomposite did not show any cytotoxic effect on NIH 3T3 cells, which act as good biocompatibility agent. Furthermore, GP@GO-KR nanocomposite promoted the wound healing efficiency in the mouse by stimulating the tissue regeneration. It is concluded that the nanocomposite produced in this study may be used as a potential bactericidal and wound healing agent in pharmaceuticals.