▎ 摘　　要

A novel electrochemiluminescence (ECL) biosensor based on platinum nanoflowers (PtNFs)/graphene oxide (GO)/glucose oxidase (GODx) was discovered for glucose detection. PtNFs/GO was synthesized using a nontoxic, rapid, one-pot and template-free method and characterized by transmission electron microscopy (TEM) and high-resolution TEM techniques. The as-prepared PtNFs/GO with clean surface and multiporous structure was used to assemble GODx to form a glucose biosensor. Based on ECL results, the PtNFs/GO/GODx film-modified electrode displayed a high electrocatalytic activity towards the oxidation of glucose, which generated hydrogen peroxide (H2O2) to react with the luminol radicals thus enhanced the luminol ECL. Under the optimized conditions, two linear regions of ECL intensity to glucose concentration were valid in the range from 5 to 80 mu mol/L (r = 0.9957) and 80 to 1,000 mu mol/L (r = 0.9909) with a detection limit (S/N = 3) of 2.8 mu mol/L. In order to verify the reliability, the thus-fabricated biosensor was applied to determine the glucose concentration in glucose injection, glucose functional drink, and blood serum. The results indicated that the proposed biosensor presented good characteristics in terms of high sensitivity and good reproducibility for glucose determination, promising the applicability of this sensor in practical analysis.