• 文献标题:   Fluorescent sensing of cocaine based on a structure switching aptamer, gold nanoparticles and graphene oxide
  • 文献类型:   Article
  • 作  者:   SHI Y, DAI HC, SUN YJ, HU JT, NI PJ, LI Z
  • 作者关键词:  
  • 出版物名称:   ANALYST
  • ISSN:   0003-2654 EI 1364-5528
  • 通讯作者地址:   Changchun Inst Appl Chem
  • 被引频次:   38
  • DOI:   10.1039/c3an00897e
  • 出版年:   2013

▎ 摘  要

This study demonstrates a cocaine sensing method employing graphene oxide (GO), gold nanoparticles and a structure switching aptamer, which can fold into a three-way junction in the presence of cocaine. On the observation of gold nanoparticles (Au NPs) induced graphene oxide fluorescence quenching, a structure switching aptamer of cocaine was introduced as the linker between the two parts. Firstly, two fragments of a cocaine aptamer were immobilized covalently onto GO and Au NPs, respectively. Then when the three-way junction formed, the Au NPs were drawn near to the GO surface and induced a fluorescence intensity decrease. The limit of detection was 0.1 mu M for cocaine in purified water, and well defined results were also obtained in biological fluids and the specificity experiment, which expands the feasibility of the as-prepared sensor for practical applications.