• 文献标题:   Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of beta-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties
  • 文献类型:   Article
  • 作  者:   ORFANAKIS G, PATILA M, CATZIKONSTANTINOU AV, LYRA KM, KOULOUMPIS A, SPYROU K, KATAPODIS P, PAIPETIS A, RUDOLF P, GOURNIS D, STAMATIS H
  • 作者关键词:   bioconjugate, immobilization, graphene, magnetic nanoparticle, nanoscaffold
  • 出版物名称:   FRONTIERS IN MATERIALS
  • ISSN:   2296-8016
  • 通讯作者地址:   Univ Ioannina
  • 被引频次:   6
  • DOI:   10.3389/fmats.2018.00025
  • 出版年:   2018

▎ 摘  要

Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of beta-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70 degrees C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles.