▎ 摘　　要

We have constructed a glucose/O-2 biofuel cell (BFC) with electrochemically reduced graphene oxide - multiwalled carbon nanotube (ERGO-MWCNT) modified glassy carbon electrode (GCE) as anode and graphene-Pt composite modified GCE as cathode. The electrochemical characterization results show that enzyme GOx was well immobilized onto the composite modified electrode. Moreover the composite modified film exhibits excellent catalytic ability towards the oxidation of glucose in the presence of redox mediator hydroquinone (HQ). Graphene -Pt composite has been prepared by simple sodium borohydride reduction method and characterized. The graphene-Pt composite modified GCE shows good electrocatalytic activity towards O-2 reduction. A membraneless glucose/O-2 biofuel cell (BFC) has been developed by employing ERGO-MWCNT modified GCE as anode and graphene-Pt as cathode. The maximum power density of 46 mu W cm(-2) was achieved for the constructed biofuel. The results showed that graphene based composites are potential candidates for the development of efficient biofuel cells.