• 文献标题:   A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite
  • 文献类型:   Article
  • 作  者:   SHAFIEI F, SABERI RS, MEHRGARDI MA
  • 作者关键词:   cancer cell, aptamer, graphene, chitosan, gold nanoparticle
  • 出版物名称:   BIOELECTROCHEMISTRY
  • ISSN:   1567-5394 EI 1878-562X
  • 通讯作者地址:  
  • 被引频次:   25
  • DOI:   10.1016/j.bioelechem.2021.107807 EA APR 2021
  • 出版年:   2021

▎ 摘  要

Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction. Therefore, there is a great demand for fast, inexpensive, and selective detection methods. Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection. A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization. The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area. After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor. The aptasensor fabrication steps were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances. This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 x 10(1)-1 x 10(6) cells/mL, and a low detection limit of 4 cells/mL. (C) 2021 Elsevier B.V. All rights reserved.