• 文献标题:   Microfluidic electrochemical magnetoimmunosensor for ultrasensitive detection of interleukin-6 based on hybrid of AuNPs and graphene
  • 文献类型:   Article
  • 作  者:   ZHANG CY, SHI DM, LI XY, YUAN J
  • 作者关键词:   magnetoimmunosensor, electrochemical biosensor, aunp, graphene hybrid nanomaterial, integrated microfluidic device
  • 出版物名称:   TALANTA
  • ISSN:   0039-9140 EI 1873-3573
  • 通讯作者地址:  
  • 被引频次:   7
  • DOI:   10.1016/j.talanta.2021.123173 EA JAN 2022
  • 出版年:   2022

▎ 摘  要

Cytokines are important factors in the early diagnosis of autoimmune diseases and require high sensitivity, high selectivity and quantitative detection. We proposed a miniaturized electrochemical magneto-immunosensor (EC-MIS) on portable interleukin-6 (IL-6) detection based on this requirement. Firstly, a micro-fabricated working electrode is electrochemically modified with a hybrid of reduced graphene oxide (rGO) and gold nanoparticles (AuNPs). Increased surface area and enhanced charge transfer rate improve the performance of this immuno-sensor on sensitivity. Secondly, magnetic beads attached with the capture antibody (cAb) are employed in sandwich immunoassay. This kind of immunoassay is immobilized on the working electrode surface by an external magnet to enrich the analyte IL-6. Thirdly, the last two features are combined and integrated on a microfluidic device in order to restrict the sample at certain areas and ease the operation of detection. With our prototypic EC-MIS operated in amperometric mode, we have achieved the detection of IL-6 with a linear range from 0.97 to 250 pg/mL and a limit of detection (LOD) of 0.42 pg/mL. Real serum samples were demonstrated and compared with benchtop equipment's results.