▎ 摘　　要

Herein we present beta-cyclodextrin (CD)-functionalized reduced graphene oxide (RGO) nanosheets supported on silicate sol-gel matrix-embedded gold nanoparticles (Au NPs) modified electrode as a new affinity binding nanocomposite. The modified electrode is fabricated through layer-by-layer drop casting followed by immobilization of chemically modified enzyme conjugate (horse radish peroxidase (HRP)-adamantane carboxylic acid (ADA)). This affinity system is based on the supramolecular association between CDs and HRP-ADA and is mimicking the biological avidin-biotin interactions. CDs-functionalized RGO (RGO-CD) functions as a macrocyclic host to form stable supramolecular inclusion complexes with enzyme conjugate. Besides Au NPs improve the interfacial interaction with RGO-CD nanosheets, and thus exhibit synergistic electrocatalytic effect toward H2O2 reduction in the presence of 1 mM hydroquinone.