▎ 摘　　要

In this work, we first designed and synthesized an IMAC material with He4+ immobilized on poly dopamine-coated magnetic graphene and applied it to phosphopeptides enrichment successfully. The newly prepared material gathered the advantages of large specific surface area of graphene, good hydrophilicity and biocompatibility of polydopamine and superparamagnetism of Fe3O4. We investigated the performance of Hf4+-immobilized polydopamine-coated magnetic graphene (denoted as magG@PDA-Hf4+) in phosphopeptides enrichment and the results showed high selectivity and sensitivity of the new material. Finally, we successfully applied magG@PDA-Hf4+ to phosphopeptides enrichment from non-fat milk digests and human serum, further demonstrating excellent performance of this new material in phosphopeptides enrichment. (C) 2015 Elsevier B.V. All rights reserved.