▎ 摘　　要

Graphene oxide (GO) and Au Nanoparticles (NPs) hybrid hydrogels were prepared through one-step hydrothermal method. The microstructure of GO/AuNPs hydrogels determined by SEM demonstrated that the hydrogels displayed three-dimensional (3D) network structure. The freeze-dried GO/AuNPs hydrogels (xerogels), i.e., GO/Au nanocomposites, could efficiently catalyze the oxidation reaction of peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB), in the presence of H2O2 to produce a blue color reaction. Compared with the natural enzyme, horse radish peroxidase (HRP), GO/Au nanocomposites exhibited higher peroxidase-like activity. The reason might be that the porous 3D network structure of GO/Au nanocomposite enhanced the reaction activity. The catalytic reaction mechanism of GO/Au nanocomposite was followed the Michaelis-Menten behavior. The combination of GO/Au nanocomposite with glucose oxidase (GOx) performed a selective colorimetric assay for glucose detection with a detection limit of 0.4728 mu mol.L-1, which can be used as a promising candidate biosensor for glucose detection.