▎ 摘　　要

Holey graphene oxide (HGO) has been synthesized by one step hydrothermal method and applied to construct a highly sensitive acetylcholinesterase (AChE) biosensor. The developed system is characterized by using SEM, TEM, nitrogen adsorption-desorption, Raman, and electrochemical impedance spectroscopy. It is proved that HGO can effectively immobilize AChE and accelerate electron transfer rate. The prepared biosensor AChE/HGO/glass carbon electrode (GCE) shows excellent affinity to the acetylthiocholine chloride (ATCl) with a Michaelis-Menten constant value of 0.23mM. Under optimum conditions, AChE/HGO/GCE can effectively detect paraoxon with a linear ranged from 10 to 45ng/mL and a detection limit of 1.58ng/mL. Furthermore, the prepared AChE/HGO/GCE biosensor demonstrates excellent stability and reproducibility, which provides a reliable method for the detection of organophosphate pesticides. The developed biosensor is used for detecting paraoxon in real samples of lettuce and cabbage and shows a satisfactory recovery.