▎ 摘　　要

Here, we report the efficient loading of Juniperus squamata root essential oil (JSEO) on polyvinylpyrrolidone (PVP) functionalized graphene oxide (GO-PVP) and investigated cytotoxic potential of GO-PVP-JSEO against human breast cancer cells MDA-MD-231. For this, modified Hummer's method was used to synthesize GO followed by its functionalization with PVP. Loading of JSEO on nanocarriers i.e., GO and GO-PVP was carried out through the pi-pi/hydrophobic interactions and we found that GO-PVP (17.4%) exhibit higher JSEO loading capacity in comparison to GO (13.6%). Further, GC and GC-MS techniques were used to analyze biologically active phytochemicals, which enabled the identification of sixty-four (64) compounds. To characterize the synthesize nanocomposites, latest spectroscopic techniques viz. Raman, FT-IR, XRD, SEM were used. Moreover, the cell survival percentages of GO-PVP-JSEO treated MDA-MD-231 human breast cancer cells at 20 mu g/mL concentration were about 68%, 32.74%, and 21%, after 24 h, 48 h and 72 h, respectively. These results reveal the enhanced anticancer activity of GO-PVP-JSEO nanocarrier in comparison to JSEO alone. Overall, our novel finding shows that JSEO loaded GO-PVP nanocarrier could be a promising platform for breast cancer therapy. Also, future pre-clinical and clinical studies are urgently needed to evaluate the safety and efficacy of GO-PVPJSEO for treating breast cancer.