▎ 摘　　要

The rapidly increasing application and production of graphene oxide (GO) may lead to its environmental release at high levels. Haplic Cambisols with Larixolgensis seedlings were treated with different concentrations of GO (0, 25, 50, 150, 250, and 500 mg L-1, expressed as CK, T1, T2, T3, T4, and T5, respectively) for 50 days. Then, we investigated the influence of GO concentrations on the bacterial community of rhizospheric soil by High-throughput sequencing technology. Adding GO significantly increased bacterial community richness, with a trend of increasing initially, decreasing and then increasing with increasing GO concentration. The diversity indices differed by treatments, but the pattern of change was not obvious. Except for a few unidentified bacteria, the relative abundances of Acidobacteria, Actinobacteria, Chloroflexi, Nitrospirae, Proteobacteria and Verrucomicrobia were the highest, and different concentrations of GO changed the relative abundance of the main bacterial populations. The rank order of bacterial similarity was T5 = T1 > T2 = T4 > T3 > CK. Different GO treatments usually significantly enhanced the metabolic function of bacteria. This empirical study is the first to provide experimental evidence revealing the interrelations between GO contamination and changes of bacterial community diversity of Larixolgensis rhizospheric Haplic Cambisols in Northeast China.