▎ 摘　　要

A novel voltammetric sensor was developed for the determination of tobramycin by combining the excellent electronic conductivity of reduced graphene oxide (rGO) and the signal amplification properties of graphene oxide (GO). Compared with glassy carbon (GC), rGO-modified GC, and GO-modified GC electrodes, rGO/GO hybrid-modified GC electrode exhibited higher electrocatalytic activity toward the tobramycin electro-oxidation, with a more well-defined peak shape. The electro-oxidation peak current was found to be proportional to the tobramycin concentration in two ranges of 7.0-50.0 and 50.0-900.0M, with a detection limit of 2.0M. To control and reduce dose-dependent toxicity of aminoglycoside drugs, an accurate determination of their concentration in blood is important. While some methods have been reported so far for determination of tobramycin, as an aminoglycoside-type drug, it is vital to develop simple, sensitive, inexpensive, and fast methods for its assay in human blood samples to control its dose-dependent toxic side effects. Therefore, the above developed method was applied in the analysis of tobramycin in spiked human blood serum samples. The recovery results were satisfactory.