▎ 摘　　要

A novel electrochemical active electrode based on isopolyacid ion IMo6O245- (IMo6), Pt and graphene oxide (GO) had been fabricated, which was used as a biosensor in double signal amplification platform for simultaneous detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Cyclic voltammetry, amperometry and differential pulse voltammetry indicated that a glass carbon electrode modified with Pt/IMo6/GO (Pt/IMo6/GO-GCE) presented higher electrochemical activity toward the oxidation of AA, DA and UA and faster response than those of a bare GCE, GO-GCE, Pt/GO-GCE and IMo6/GO-GCE. As an electrochemical sensor, the Pt/IMo6/GO-GCE showed a wide linear range of 50-4000 mu M, 4-750 mu M, and 75-300 mu M with the limits of detection (S/N = 3) as 6.4 mu M, 0.22 mu M and 0.72 mu M, corresponding to AA, DA and UA, respectively. In addition, the Pt/IMo6/GO-GCE provided a new option in the simultaneous determination of AA, DA and UA in human blood serum with reliable recovery. (C) 2019 The Electrochemical Society.