• 文献标题:   Simultaneous voltammetric determination of guanine and adenine using MnO2 nanosheets and ionic liquid-functionalized graphene combined with a permeation-selective polydopamine membrane
  • 文献类型:   Article
  • 作  者:   ZHANG S, ZHUANG XM, CHEN DD, LUAN F, HE T, TIAN CY, CHEN LX
  • 作者关键词:   electrochemical, nanocomposite, sensor, guanine, adenine, fetal bovine serum, mouse whole blood
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Yantai Univ
  • 被引频次:   17
  • DOI:   10.1007/s00604-019-3577-4
  • 出版年:   2019

▎ 摘  要

Guanine and adenine in blood samples can be detected by using an electrochemical sensor based on the use of manganese dioxide (MnO2) nanosheets and ionic liquid functionalized graphene (IL-GR) bound to a polydopamine (PDA) membrane. Both guanine and adenine undergo aredox reaction on the surface of the modified electrode. Cyclic voltammetry and differential pulse voltammetry were used to evaluate the electrochemical behavior of a glassy carbon electrode (GCE) modified with PDA/MnO2/IL-GR. The sensor allows for individual as well as simultaneous determination of guanine and adenine. The working voltage of differential pulse voltammetry at which data were acquired to establish the calibration plot: 0.6-1.2V for guanine, 0.8-1.4V for adenine, 0.4-1.4V for mixture of guanine and adenine. A wide detection range (10-300M), low detection limits (guanine: 0.25M; adenine: 0.15M), selectivity and reproducibility are demonstrated. The modified GCE was successfully applied to the analysis of guanine and adenine in spiked fetal bovine serum and mouse whole blood samples.