▎ 摘　　要

The hydrogels based on natural polysaccharides enjoy from the biocompatibility and economic favorites. In this work, mucilage of Ocimum basilicum seeds was extracted and used for the preparation of hydrogels followed by study of the hydrogel properties. The hydrogels were prepared by graft copolymerization of acrylic acid and acrylamide monomers to the polysaccharide chains extracted from O. basilicum seeds. Graphene Oxide (GO) nanosheets, produced by the green, easy and cost-effective electrochemical exfoliation method, were used to improve the physical and mechanical properties of prepared natural-based hydrogels. FTIR spectroscopy, SEM images and XRD analysis were used to characterize the prepared hydrogels. The structure and water absorption properties of hydrogels based on O. basilicum Mucilage (OBM), grafted by acrylic acid (AA) and acrylamide (AAm) copolymers (OBM-g-poly(AA-co-AAm)), were investigated and the effect of GO additive was evaluated. GO nanosheets, alters the porosity and water absorption behavior of OBM based hydrogels and in 1.25% wt of GO additive, the highest water absorption rate was obtained which is due to the suitable porosity of the hydrogel and the higher contact area between the water molecules and the hydrogel structure. More studies showed the increasing of pH and saline solution sensitive responsibility of OBM based hydrogel by incorporating GO nanosheet having highly hydrophilic functional groups. Finally, rheological studies showed that by increasing the amount of GO content, the hydrogels got stiffer, which is due to their highly compressed structure. This is attributed to the more functional groups present on GO nanosheets and their physical and chemical crosslinking effect in the formation of hydrogel networks with a more compact structure. Considering the advanced physical and mechanical properties of the novel OBM based nanocomposite hydrogels, they have high potential for use in biomedical applications such as tissue engineering scaffolds, wound dressing films and drug delivery systems.