▎ 摘　　要

Introducing textures and coatings to titanium alloy implant is conducive to improving biocompatibility. In this paper, a two-step scheme comprising laser texturing process followed by electrophoretic deposition stage was investigated to fabricate micron-scale grooves covered with nano-scale graphene oxide (GO) coating precisely and controllably. The effect of multiple-pulse laser ablation on Ti6Al4V alloy (TC4) was analyzed and the correlation between laser parameters and ablation size was built. The normalized pulse overlap was adjusted by changing scanning speed and laser repetition frequency to fabricate well-structured and controllable microgrooves. Electrophoretic deposition (EPD) of GO was conducted onto micro-grooved TC4 substrate to further improve the biocompatibility. The electrophoretic deposition process was conducted with different graphene oxide concentration and voltages at deposition times from 10 to 25 min. Thereafter, SEM, XRD and Raman test were carried out to analyze the quality and morphology of coatings. In addition, in vitro cellular evaluations and immunofluorescence staining had been used to test the biological properties of the micro-nano structure. The study shows that the micron-scale groove structure and nano-scale GO coating can remain the advantage of groove structure in cell contact guidance, as well as the advantage of GO in biocompatibility, which can promote cell adhesion and proliferation.