▎ 摘　　要

Horseradish peroxide was coupled firmly to graphene oxide with Fe3O4@SiO(2)tethering via synergy of physical adsorption, chemical bonding as well as adjacent complexation and enzyme based electrode was prepared on the basis of this nano-complex. Conglomeration modality, configuration of cofactor in redox protein, physiochemical properties of nano-composite with protein integration and mutual interactions between protein supporter and incorporated heme protein were characterized and investigated by spectrometric, microscopic and electro-chemical techniques. Results from experiments revealed that interactions between immobilized redox protein and enzyme carrier would lead to partial ordered array of attached protein molecules with quasi-spherical shape. Such mutual interactions would also improve hydrophilicity of redox protein molecules anchoring on nano-composite to some extent via the formation of amphiphilic micelle-like agglomeration. Mild combination of redox protein with element of nano-composite as fluorescence quenching ligand would hoist the active energy of charge shuttle to some extent. Direct electron shuttle of heme protein based electrode should be ascribed to quasi reversible surface-confined process with one proton and single electron involved. As-prepared enzyme based electrode displayed favorable catalytic performance on electro-reduction of H2O2. Onset-potential of electro-reduction was similar to 0.04 V and apparent turn-over frequency of substrate was figured out to be 0.17 s(-1). This enzyme based electrode exhibited excellent sensing function to substrate (K-M: 1.13 mM), desirable detection limit (3.1 mu M) and moderate sensitivity (5.05 x 10(-3)mu A mu M-1). Graphic Abstract Illustration in procedure of magnetic Fe3O4 core-SiO2 shell nanoparticle-graphene oxide composite preparation and mutual interactions between elements in nano-complex and immobilized horseradish peroxide. [GRAPHICS]