• 文献标题:   Self-assembled covalent capillary coating of diazoresin/carboxyl fullerene for analysis of proteins by capillary electrophoresis and a comparison with diazoresin/graphene oxide coating
  • 文献类型:   Article
  • 作  者:   YU B, SHU X, CONG HL, CHEN X, LIU HW, YUAN H, CHI M
  • 作者关键词:   capillary electrophoresi, capillary coating, diazoresin, carboxyl fullerene, protein
  • 出版物名称:   JOURNAL OF CHROMATOGRAPHY A
  • ISSN:   0021-9673 EI 1873-3778
  • 通讯作者地址:   Qingdao Univ
  • 被引频次:   24
  • DOI:   10.1016/j.chroma.2016.02.001
  • 出版年:   2016

▎ 摘  要

Self-assembled and covalently linked capillary coatings of carboxyl fullerenes (C-60-COOH) were prepared using photosensitive diazoresin (DR) as a coupling agent. Layer by layer (LBL) self-assembled DR/C-60-COOH coatings based on ionic bonding was fabricated first on the inner surface of silica capillary, and subsequently converted into covalent bonding after treatment with UV light through a unique photochemistry reaction of DR. The covalently bonded coatings had the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus the baseline separation of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and myoglobin (Mb) was achieved within 13 min by using capillary electrophoresis (CE). The covalently linked DR/C-60-COOH capillary coatings presented good chemical stability and repeatability. The reproducibility of the separation of proteins was less than 1%, 2.5%, and 3.5%, respectively, for run-to-run, day-to-day, capillary-to-capillary, respectively; and the RSD of migration time for the proteins are all less than 2.5% after a continuous 100 times running in a coating column. Compared with DR/graphene oxide (GO) coatings prepared by the same method, the DR/C-60-COOH capillary coatings showed excellent protein separation performance due to a self lubrication based anti-fouling mechanism. Because of the replacement of highly toxic and moisture sensitive silane coupling agent by DR in the covalent coating preparation, this method may provide an environmentally friendly and simple way to prepare the covalently coated capillaries for CE. (C) 2016 Elsevier B.V. All rights reserved.