▎ 摘　　要

Purpose Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed. Design/methodology/approach Here degrees C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1 degrees C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles degrees 8rGO-MNPS degrees 9 and leading to effective fluorescence quenching. Upon adding PD-L1 degrees C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed degrees C resulting in the recovery of quenched fluorescence. Findings This sensor can detect PD-L1 with a linear range from 100 pg mL(-1) to 100 ng mL(-1), and a detection limit of 10 pg center dot m(-1) was achieved. Originality/value This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors.