• 文献标题:   Highly Sensitive Electrochemical Aptasensor for Detection of Glypican-3 Using Hemin-Reduced Graphene Oxide-Platinum Nanoparticles Coupled with Conductive Reduced Graphene Oxide-Gold Nanoparticles
  • 文献类型:   Article
  • 作  者:   LI GY, LI HM, CHEN W, CHEN HJ, WU GX, TAN MX, LIANG JT, ZHOU ZD
  • 作者关键词:   electrochemical aptasensor, glypican3, aptamer, differential pulse voltammetry
  • 出版物名称:   JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
  • ISSN:   1550-7033 EI 1550-7041
  • 通讯作者地址:  
  • 被引频次:   2
  • DOI:   10.1166/jbn.2021.3215
  • 出版年:   2021

▎ 摘  要

An electrochemical aptasensor for quantitatively detecting glypican-3 (GPC3) was constructed by combining hemin-reduced graphene oxide-platinum (H-rGO-Pt) nanoparticles (NPs) with reduced graphene oxide-gold (rGO-Au) nanoparticles (NPs). Herein, the rGO-Au NPs deposited onto screen-printed electrodes resulted in signal amplification due to their large surface areas. Meanwhile, highly conductive H-rGO-Pt NPs acted as a sensing medium that improved electrical conductivity and as an indicator for monitoring peak current for determination. A GPC3 aptamer (GPC3apt) with a low equilibrium dissociation constant was used as a bio-recognition molecule. GPC3apt specifically captured GPC3 proteins and formed aptamer-GPC3 complexes, which impeded electron transfer and thus hampered the redox signal of hemin in H-rGO-Pt NPs. This developed electrochemical aptasensor showed a linear response to GPC3 (from 0.001 mu g/mL to 10 mu g/mL) and had a detection limit of 0.001 mu g/mL. This work provides a low-cost and highly sensitive detection with and good recovery for GPC3 and holds great promise for the clinical diagnosis of hepatocellular carcinoma.