• 文献标题:   Synthesis of the multi-walled carbon nanotubes-COOH/graphene/gold nanoparticles nanocomposite for simple determination of Bilirubin in human blood serum
  • 文献类型:   Article
  • 作  者:   FENG QL, DU YL, ZHANG C, ZHENG ZX, HU FD, WANG ZH, WANG CM
  • 作者关键词:   bilirubin, carbon material, biosensor, aunp
  • 出版物名称:   SENSORS ACTUATORS BCHEMICAL
  • ISSN:   0925-4005
  • 通讯作者地址:   Lanzhou Univ
  • 被引频次:   37
  • DOI:   10.1016/j.snb.2013.05.035
  • 出版年:   2013

▎ 摘  要

In this work, the multi-walled carbon nanotubes-COOH (MWCNTs-COOH)/graphene (GNs)/gold nanoparticles (AuNPs) composite materials were synthesized by in situ growth of AuNPs on the surface of the MWCNTs-COOH/GNs. And then, the Bilirubin oxidase (BUD) was immobilized on the surface of the film to construct a biosensor for determination of Bilirubin. Morphology of the nanomaterials was analyzed by field-emission scanning electron microscopy, high resolution transmission electron microscopy, Brunauer-Emmett-Teller, and X-ray photoelectron spectroscopy. The performance of the biosensor was studied by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and amperometric response (i-t). It was demonstrated that the nanocomposite materials exhibited enhanced fast electron transfer and excellent biocompatibility. In this assay, the MWCNTs-COOH/GNs/AuNPs composite film based biosensor showed prominently electrocatalytic activity for the detection of Bilirubin with a linear range from 1.33 mu M to 71.56 mu M and the low limit of detection (LOD) is 0.34 mu M based on a signal to noise ratio (S/N = 3). Meanwhile, the apparent Michaelis-Menten constant (K-m) was 64.86 mu M. In addition, this biosensor exhibited satisfying reproducibility, stability, feasibility and fast responses. These results provide a novel way for exact detection of Bilirubin in biotechnology and clinical diagnosis, and broaden the application of graphene and carbon nanotubes in biomaterials. (C) 2013 Elsevier B.V. All rights reserved.