▎ 摘　　要

GO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction analysis (XRD), Raman spectroscopy, Thermo-gravimetric analysis (TGA), X-ray fluorescence (XRF), Zeta potential analysis, Scanning electron microscopy (SEM), and Atomic force microscopy (AFM) techniques were used to reveal that AgNPs have covered the surface of GO sheets through non covalent and permanent bonding, altering new structural and electronic properties, leading to the appearance of the oxidative stress phenomenon, which considered as a key step of the antibacterial mechanism of this kind of hybrids, causing death of both Gram-positive (Bacillus subtilis, Enterococcus faecalis, Methicillin-resistant Staphylococcus aureus - MRSA, and Staphylococcus aureus) and Gram-negative (Escherichia coli, Serratia marcescens, Shigella sp, Salmonella sp, Serratia microorganisms liquefaciens, Proteus sp, Enterobacter cloacae and Pseudomonas aeruginosa).