• 文献标题:   Aptamer based voltammetric biosensor for Mycobacterium tuberculosis antigen ESAT-6 using a nanohybrid material composed of reduced graphene oxide and a metal-organic framework
  • 文献类型:   Article
  • 作  者:   LI LL, YUAN YH, CHEN YJ, ZHANG P, BAI Y, BAI LJ
  • 作者关键词:   aptasensor, pt@au nanoparticle, cyclic voltammetry, electrochemical impedance spectroscopy, signal amplification, electrochemical detection, effective surface area
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Chongqing Med Univ
  • 被引频次:   8
  • DOI:   10.1007/s00604-018-2884-5
  • 出版年:   2018

▎ 摘  要

The 6-kDa early secretory antigenic target referred to as ESAT-6 is a virulence factor secreted by Mycobacterium tuberculosis (MTB). This work describes a voltammetric aptasensor for ultrasensitive detection of ESAT-6. Reduced graphene oxide doped with metal-organic framework (MOF-rGO) was deposited on a glassy carbon electrode (GCE). This increases the immobilization of electroactive Toluidine Blue (TB) and facilitates the electron transfer from TB to the modified GCE. Platinum/gold core/shell (Pt@Au) nanoparticles were used to assemble thiolated ESAT-6 binding aptamer (EBA) on a modified electrode and to further amplify the response to TB. The modified GCE, typically operated at -0.36 V (vs. SCE), has a linear response in 1.0 x 10(-4) to 2.0 x 10(2) ngai...mL(-1) ESAT-6 concentration range, and the limit of detection (LOD) for ESAT-6 is as low as 3.3 x 10(-5) ngai...mL(-1). It exhibits satisfactory specificity and reproducibility when analyzing spiked human serum.