▎ 摘　　要

Pyrazinamide (PZN) also called as Pyrazinecarboxamide is a widely used drug for the treatment of Tuberculosis. In the present work, we proposed a sensitive method for determination of PZN using Graphene-Zinc oxide-Carbon paste electrode (GNS-ZnO-CPE). The surface characterization of electrode materials was done by using X-Ray diffraction, Scanning electron microscopy, Energy dispersive X-ray spectroscopy and transmission electron microscopy. The electrocatalytic response of PZN at GNS-ZnO-CPE was measured using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). In addition, electrochemical impedance studies revealed that the smaller R-ct value was observed at GNS-ZnO-CPE as compared to that of CPE, which authenticates its good conductivity. Under the optimized conditions, I-p (mu A) was proportional to PZN concentration in the range of 1.5 x 10(-7) to 4.0 x 10(-4) M with a detection limit of 4.31 x 10(-8) M. The proposed electrochemical sensor showed excellent recovery in pharmaceutical formulations, urine and blood serum samples which revealed the promising practicality of sensor for PZN detection. (C) 2016 Elsevier B.V. All rights reserved.