▎ 摘　　要

Pathogenic bacterial infection has been becoming a global threat toward people's health, especially the massive usage of antibiotics due to the lack of antibacterial agents with less side effects. Developing new nanoagents to fight pathogenic bacteria has provided enormous new possibilities in the treatment of bacterial infections, such as graphene-based two-dimensional (2D) antibacterial nanoagents with different bacterial inhibition capabilities; however, mussel-inspired design of near-infrared (NIR)-responsive and biocompatible Ag-graphene nano agents possessing efficient and versatile bacterial disinfection activities have rarely been reported. In this study, we developed a new kind of antibacterial nanoagent, dopamine-conjugated polysaccharide sulfate-anchored and-protected Ag-graphene (Ag@G-sodium alginate sulfate ((SAS)) nanocomposite, to combat bacterial infection and contamination in different application fields. Ag@G-SAS exhibited robust antibacterial activity toward both Escherichia coli and Staphylococcus aureus; notably, the nanoagent can significantly inhibit S. aureus infection on wounded pig skin without or with NIR laser. Besides wound disinfection, the 2D Ag@G-SAS can also serve as a good layer-by-layer (LbL) building block for the construction of self-sterilizing coatings on biomedical devices. All of the results verified that the LbL-assembled Ag@G-SAS coating exhibited favorable bactericidal activity, extraordinary blood compatibilities, and good promotion ability for cell proliferation. Owing to the shielding effects of heparin-like polysaccharide sulfates, the Ag@G-SAS nanoagent showed limited cytotoxicity toward mammalian cells. Combining all of the advantages mentioned above, it is believed that the proposed Ag@G-SAS nanoagent and its LbL-assembled coatings may have versatile application potentials to avoid bacterial contaminations in different fields, such as wounded skin, disinfection of biomedical implants and devices, and food packaging sterilization.