▎ 摘　　要

Bone defects are major challenges that must be rapidly treated. The preparation of scaffolds is an appropriate strategy for the treatment of defects. This study aimed to characterize and apply of a scaffold containing chitosan, alginate, selenium and graphene nano-oxide (CAHG-Se) for bone regeneration in a rabbit model. FT-IR, FE-SEM, TEM, XRD, TGA and DTG were used to investigate the properties of the prepared scaffolds. We also investigated porosity, density, water absorption and retention, mechanical properties and cell viability of the scaffolds. Scaffolds were also used in a rabbit model and radiographs were prepared. SEM images showed that introducing graphene increased porosity and density. The results showed good safety in all the scaffolds. Introducing sele-nium and graphene nano-oxide could significantly increase water absorption and retention and mechanical properties of the scaffolds compared with scaffolds lacking them (P<0.05). The results for clinical results showed that rabbits treated with scaffolds prepared from CAHG-Se significantly regenerated bone defects compared control group (P<0.05). In conclusion, selenium and graphene nano-oxide have synergistic effects on the regeneration of bone defects that must be evaluated in future studies.