• 文献标题:   A novel ECL biosensor for the detection of concanavalin A based on glucose functionalized NiCo2S4 nanoparticles-grown on carboxylic graphene as quenching probe
  • 文献类型:   Article
  • 作  者:   LI XJ, WANG YG, SHI L, MA HM, ZHANG Y, DU B, WU D, WEI Q
  • 作者关键词:   electrochemiluminescence biosensor, quenching, chitosan/ru bpy 3 2+ /silica/fe3o4 nanomaterial, glucose functionalized nico2s4 nanoparticles grown on carboxylic graphene, specific carbohydratecon a interaction
  • 出版物名称:   BIOSENSORS BIOELECTRONICS
  • ISSN:   0956-5663 EI 1873-4235
  • 通讯作者地址:   Univ Jinan
  • 被引频次:   69
  • DOI:   10.1016/j.bios.2017.04.050
  • 出版年:   2017

▎ 摘  要

An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy)(3)(2+)/silica/Fe3O4 nanomaterials (CRuSi-Fe3O4) were synthesized through W/O microemulsion route. The added Fe3O4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe3O4. In addition, the layered structure of CRuSi-Fe3O4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo2S4 nanoparticles- grown on carboxylic graphene (NiCo2S4-COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo2S4 was used to quench the ECL of CRuSi-Fe3O4, graphene was used to grow NiCo2S4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S2O82- as the coreactant of CRuSi-Fe3O4. Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL(-1) to 100 ng mL(-1) was obtained, and the detection limit was calculated to be 0.18 pg mL(-1) (S/ N = 3).