• 文献标题:   Amplified detection of leukemia cancer cells using an aptamer-conjugated gold-coated magnetic nanoparticles on a nitrogen-doped graphene modified electrode
  • 文献类型:   Article
  • 作  者:   KHOSHFETRAT SM, MEHRGARDI MA
  • 作者关键词:   ccrfcem, cancer cell, nitrogendoped graphene, aptamer, gold coated magnetic nanopartide
  • 出版物名称:   BIOELECTROCHEMISTRY
  • ISSN:   1567-5394 EI 1878-562X
  • 通讯作者地址:   Univ Isfahan
  • 被引频次:   28
  • DOI:   10.1016/j.bioelechem.2016.12.001
  • 出版年:   2017

▎ 摘  要

The increasing demands for early, accurate and ultrasensitive diagnosis of cancers demonstrate the importance of the development of new amplification strategies or diagnostic technologies. In the present study, an aptamer-based electrochemical biosensor for ultrasensitive and selective detection of leukemia cancer cells has been introduced. The thiolated sgc8c aptamer was immobilized on gold nanoparticles-coated magnetic Fe3O4 nanoparticles (Apt-GMNPs). Ethidiunl bromide (EB), intercalated into the stem of the aptamer hairpin, provides the read-out signal for the quantification of the leukemia cancer cells. After introduction of the leukemia cancer cells onto the Apt-GMNPs, the hairpin structure of the aptamer is disrupted and the intercalator molecules are released, resulting in a decrease of the electrochemical signal. The immobilization of nitrogen-doped graphene nanosheets on the electrode surface provides an excellent platform for amplifying the read-out signal. Under optimal conditions, the aptasensor exhibits a linear response over a wide dynamic range of leukemia cancer cells from 10 to 1 x 10(6) cell mL I. The present protocol provides a highly sensitive, selective, simple, and robust method for early stage detection of leukemia cancer. Furthermore, the fabricated aptasensor was successfully used for the detection of leukemia cancer cells in complex media such as human blood plasma, without any serious interference. (C) 2016 Published by Elsevier B.V.