• 文献标题:   A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer
  • 文献类型:   Article
  • 作  者:   RAHIMZADEH Z, NAGHIB SM, ASKARI E, MOLAABASI F, SADR A, ZARE Y, AFSHARPAD M, RHEE KY
  • 作者关键词:   single cell, breast cancer, immunosensor, graphene, herceptin
  • 出版物名称:   NANOTECHNOLOGY REVIEWS
  • ISSN:   2191-9089 EI 2191-9097
  • 通讯作者地址:  
  • 被引频次:   20
  • DOI:   10.1515/ntrev-2021-0049
  • 出版年:   2021

▎ 摘  要

In this paper, we use a simple and cheap approach for the synthesis of herceptin-conjugated graphene biosensor to detect the HER2-positive breast cancer cells. The bifunctional graphene-herceptin nanosheets are prepared from graphite by a simple ultrasonic-mediated technique. The prepared protein-mediated graphene is fully characterized. The results show the exfoliation of graphene layers in herceptin solution. Moreover, herceptin is effectively conjugated into the surface of graphene nanosheets. The synthesized herceptin-conjugated graphene is applied for breast cancer detection. The linear range of this biosensor is 1-80 cells, which is significant. The biosensor shows an excellent selectivity performance for detection of HER2-positive cancer cells. Likewise, the stability and functionality of the biosensor is about 40 days. Based on the results, this device is a promising candidate for rapid and selective detection of cancer cells.