▎ 摘　　要

The significance of in situ detection of small biomolecules associated with specific cancer diseases have stimulated the design of flexible film electrode materials. In this work, a green and facile strategy was presented to fabricate flexible N,S-reduced graphene oxide film (N,S-rGOF) by respiratory mechanism of sulfate-reducing bacteria (SRB). The used microbial method allows us to fabricate N,S-rGOF under green and mild conditions. Benefiting from simultaneous double doping of N and S atoms into graphene structure, the as-obtained flexible N,S-rGOF demonstrates excellent electro-chemical sensing activity towards cancer biomarker hydrogen peroxide (H2O2) detection in terms of high sensitivity, good stability and particular reproducibility. The current response has a linear relationship with the concentration of H2O2 up to 453 mM. The detection limit is 50 mu M (S/N = 3), and the detection sensitivity is 47 mu A cm(-2) mM(-1). It is worth noting that the direct growth of breast cancer cells on N,S-rGOF can largely shorten the diffusion distance of H2O2 molecules to the active sites on N,S-rGOF surface, and therefore increases the sensitivity and accuracy of in situ detection, which offers new clinical possibilities for breast cancer diagnosis and treatment (C) 2020 Published by Elsevier B.V.