▎ 摘　　要

A Hemin-functionalized electrochemically reduced graphene oxide (H-ERGO) nanocomposite was prepared for the construction of a nonenzymatic H2O2 biosensor. H-ERGO was directly modified on the electrode surface without using a binder by electrochemical reduction. Detailed physical and electrical characterizations of H-ERGO were conducted by scanning electron microscopy, UV-vis absorption spectroscopy, and electrochemical impedance spectroscopy (EIS). Taking advantage of the superior catalytic properties of H-ERGO, we applied the proposed biosensor to H2O2 analysis. Under optimized conditions, the H-ERGO-based biosensor exhibited a wide linear range from 25 to 8850 and 8850 to 28850 mu M, with a detection limit (S/N = 3) o f 8.33 mu M f or H2O2 determination. Furthermore, it was also successfully applied to detect H2O2 in milk samples with recoveries ranging from 94.6 to 98.1%.