▎ 摘　　要

Uptake kinetics and delivery mechanisms of nano-particles (NPs) in crop plants need to be urgently understood for the application of nanotechnology in agriculture as delivery systems for eco-friendly nanoagrochemicals. Here, we investigated the uptake kinetics, translocation pathway, and key internalization process of graphene in wheat (Triticum aestivum L.) by applying three specific hydroponic cultivation methods (submerging, hanging, and split-root). Quantification results on the uptake of carbon-14 radiolabeled graphene in each tissue indicated that graphene could enter the root of wheat and further translocate to the shoot with a low delivery rate (<2%). Transmission electron microscopy (TEM) images showed that internalized graphene was transported to adjacent cells through the plasmodesmata, clearly indicating the symplastic pathway of graphene translocation. The key site for the introduction of graphene into root cells for translocation through the symplastic pathway is evidenced to be the apex of growing root hair, where the newly constructed primary cell wall is much thinner. The confirmation of uptake kinetics and delivery mechanisms is useful for the development of nanotechnology in sustainable agriculture, especially for graphene serving as the delivery vector for pesticides, genes, and sensors.