▎ 摘　　要

Bioaugmentation is considered as a promising technology for cleanup of polycyclic aromatic hydrocarbons (PAHs) from contaminated site soil, however, available high-efficiency microbial agents remain very limited. Herein, we explored graphene oxide (GO)-immobilized bacterial pellets (JGOLB) by embedding high-efficiency degrading bacteria Paracoccus aminovorans HPD-2 in alginate-GO-Luria-Bertani medium (LB) composites. Microcosm culture experiments were performed with contaminated site soil to assess the effect of JGOLB on the removal of PAHs. The results showed that JGOLB exhibited greatly improved mechanical strength, larger specific surface area and more enriched mesopores, compared with traditional immobilized bacterial pellets. They significantly increased the removal rate of PAHs by 18.51% compared with traditional bacterial pellets, reaching the removal rate at 62.86% over 35 days of incubation. Moreover, the increase mainly focused on high-molecular-weight PAHs. JGOLB not only greatly increased the abundance of embedded degrading bacteria in soil, but also significantly enhanced the enrichment of potential indigenous degrading bacteria (Pseudarthrobacter and Arthrobacter), the functional genes involved in PAHs degradation and a number of ATP transport genes in the soil. Overall, such nanocomposite bacterial pellets provide a novel microbial immobilization option for reme-diating organic pollutants in harsh soil environment.