▎ 摘 要
A highly sensitive electrochemical aptasensor is described for the determination of carcinoembryonic antigen (CEA). It is based on the use of a hybrid material composed of molybdenum selenide, graphene, and gold nanoparticles (AuNPs). The MoSe2-graphene hybrid was prepared by a hydrothermal method and used as the supporting substrate. It was placed on the surface of a glassy carbon electrode onto which the AuNPs were electrochemically deposited. Thiol-labeled aptamer against CEA was immobilized on the electrode via gold-thiol binding. The use of AuNPs coupled to the MoSe2-graphene hybrid allows for a large loading with aptamers. Under optimum conditions and at a working potential of 0.21 V (vs. SCE), the assay has a linear calibration plot in the 0.1 pg mL(-1) to 100 ng mL(-1) CEA concentration range, with a detection limit of 0.03 pg mL(-1).