• 文献标题:   Differential pulse voltammetric assay for the carcinoembryonic antigen using a glassy carbon electrode modified with layered molybdenum selenide, graphene, and gold nanoparticles
  • 文献类型:   Article
  • 作  者:   HE BS
  • 作者关键词:   twodimension transition metal dichalcogenide, ultrasensitive detection, tumor marker, aptamer, electrochemical sensor, goldthiol chemistry
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Henan Univ Technol
  • 被引频次:   10
  • DOI:   10.1007/s00604-016-2006-1
  • 出版年:   2017

▎ 摘  要

A highly sensitive electrochemical aptasensor is described for the determination of carcinoembryonic antigen (CEA). It is based on the use of a hybrid material composed of molybdenum selenide, graphene, and gold nanoparticles (AuNPs). The MoSe2-graphene hybrid was prepared by a hydrothermal method and used as the supporting substrate. It was placed on the surface of a glassy carbon electrode onto which the AuNPs were electrochemically deposited. Thiol-labeled aptamer against CEA was immobilized on the electrode via gold-thiol binding. The use of AuNPs coupled to the MoSe2-graphene hybrid allows for a large loading with aptamers. Under optimum conditions and at a working potential of 0.21 V (vs. SCE), the assay has a linear calibration plot in the 0.1 pg mL(-1) to 100 ng mL(-1) CEA concentration range, with a detection limit of 0.03 pg mL(-1).