• 文献标题:   An electrochemical aptasensor for chiral peptide detection using layer-by-layer assembly of polyelectrolyte-methylene blue/polyelectrolyte-graphene multilayer
  • 文献类型:   Article
  • 作  者:   QIN HX, LIU JY, CHEN CG, WANG JH, WANG EK
  • 作者关键词:   layerbylayer, graphene, electrochemical aptasensor, chiral peptide, aptamer
  • 出版物名称:   ANALYTICA CHIMICA ACTA
  • ISSN:   0003-2670 EI 1873-4324
  • 通讯作者地址:   Chinese Acad Sci
  • 被引频次:   36
  • DOI:   10.1016/j.aca.2011.10.044
  • 出版年:   2012

▎ 摘  要

Here we demonstrate for the first time that by physically adsorbing aptamer onto conductive film assembled via alternate adsorption of graphene/polyelectrolyte and methylene blue/polyelectrolyte, a label-free electrochemical aptasensor with high sensitivity and selectivity for peptide detection is constructed. Graphene multilayer derived from layer-by-layer assembly has played significant roles in this sensing strategy: allowing accumulation of methylene blue, facilitating electron transfer and providing much more adsorption site. As compared to previous electrochemical aptasensors, the current sensor based on graphene multilayer alternated with electroactive molecule layer offers extremely high capability for sensitive detection of target without interference of environmental surrounding. This electroactive probe-confined graphene multilayer confers great flexibility to combine with differential pulse voltammetry (DPV) together. In the presence of target D entiomer of arginine vasopressin (D-VP), the binding of peptide to aptamer block the electron transfer process of MB, leading to decreased current peak of DPV. By this way, this electrochemical aptasensor based on electroactive molecule-intercalated graphene multilayer provide highly sensitive and specific detection of D-VP with the lowest detectable concentration of 1 ng mL(-1) and a wide detection range from 1 to 265 ng mL(-1). (C) 2011 Elsevier B.V. All rights reserved.