• 文献标题:   Electrochemistry and electrocatalysis of myoglobin immobilized in sulfonated graphene oxide and Nafion films
  • 文献类型:   Article
  • 作  者:   CHEN GY, SUN H, HOU SF
  • 作者关键词:   myoglobin, sulfonated graphene oxide, electrochemistry, electrocatalysi
  • 出版物名称:   ANALYTICAL BIOCHEMISTRY
  • ISSN:   0003-2697 EI 1096-0309
  • 通讯作者地址:   Qingdao Univ
  • 被引频次:   16
  • DOI:   10.1016/j.ab.2016.03.003
  • 出版年:   2016

▎ 摘  要

In this study, sulfonated graphene oxide (SGO) was synthesized and characterized by Fourier transform infrared (FT-IR) spectroscopy and X-ray photoelectron spectroscopy (XPS). It was used to make Mb-SGO-Nafion composite films by coating myoglobin (Mb) on the glassy carbon electrodes (GCE). Positions of the Soret absorption bands suggested that Mb retained its native conformation in the films. Mb-SGO-Nafion film modified electrode showed a pair of well-defined and nearly reversible cyclic voltammetry peaks at around -0.39 V versus saturated calomel electrode (SCE) in pH 7.0 buffers, characteristic of heme Fe(III)/Fe(II) redox couples. Electrochemical parameters such as electron transfer rate constant (k(s)) and formal potential (E degrees') were estimated by fitting the data of square-wave voltammetry with nonlinear regression analysis. Experimental data demonstrated that the electron transfer between Mb and electrode was greatly facilitated and showed good electrocatalytic properties toward various substrates, such as H2O2 and NaNO2, with significant lowering of reduction overpotential. (C) 2016 Published by Elsevier Inc.