• 文献标题:   Ultrasensitive electrochemical biosensor of interferon-gamma based on gold nanoclusters-graphene@zeolitic imidazolate framework-8 and layered-branched hybridization chain reaction
  • 文献类型:   Article
  • 作  者:   BAO T, WEN MQ, WEN W, ZHANG XH, WANG SF
  • 作者关键词:   electrochemical biosensor, interferongamma, auncsgraphene@zif8, layeredbranched hybridization chain reaction, hemin/gquadruplex dnazyme, dendritic dna nanostructure
  • 出版物名称:   SENSORS ACTUATORS BCHEMICAL
  • ISSN:  
  • 通讯作者地址:   Hubei Univ
  • 被引频次:   6
  • DOI:   10.1016/j.snb.2019.05.083
  • 出版年:   2019

▎ 摘  要

In this work, a novel electrochemical biosensor was constructed for ultrasensitive interferon-gamma (IFN-gamma) detection based on graphene@zeolitic imidazolate framework-8 hybrids anchored gold nanoclusters (AuNCsGR@ZIF-8) and hemin/G-quadruplex DNAzyme decorated layered-branched hybridization chain reaction (LB-HCR). The two-dimensional GR@ZIF-8 hybrids were utilized as template to anchor AuNCs through a sequential deposition method. On the AuNCs-GR@ZIF-8 platform, an improved and efficient LB-HCR pattern was designed on the strength of traditional HCR with the cascade-like assembly of four hairpins. In which, HP1 and HP2 participated in traditional HCR process to form long double-helix DNA, AD1 and AD2 assembled alternately to facilitate the layerly chain-branching growth of DNA nanostructures. The target-triggered LB-HCR resulted in the generation of dendritic DNA nanostructures integrated with numerous in-situ formed hemin/G quadruplex DNAzyme as amplifying labels, which catalyzed the reduction of H2O2 with thionine acted as the electron mediator to realize amplified signal output. This multiple-amplified strategy exhibited good analytical performance towards IFN-gamma with a wide linear range form 1 fM to 50 pM and a low detection limit of 0.6 fM, holding promising application in disease diagnosis and monitoring.