▎ 摘　　要

The thionine-graphene (THi-GR, positively charged) nanocomposite was successfully synthesized as a good biocompatible matrix for ds-DNA which acted as a chiral selector to construct an electrochemical chiral biosensor for Tryptophan (Trp) enantiomers sensing with the assistance of Cu(ll). The nano-bionic interface was constructed as follows: Firstly, the nanocomposite was dropped on the surface of glassy carbon electrode (GCE), and then dsDNA (negatively charged) was immobilized onto the nanocomposite film via the opposite-charged adsorption techniques. This biofunctionalized nanocomposite was characterized with scanning electron microscopy (SEM), ultraviolet-visible (UV-vis) spectrometry and cyclic voltammetry (CV). The chiral biosensor was employed to study the recognition effect between ds-DNA and Trp enantiomers by CV. The results show that larger electrochemical response was obtained from L-Trp when Cu(II) was present, indicating this strategy could be employed to enantioselectively recognize Trp enantiomers. Under optimum conditions, the chiral biosensor exhibited a good linear response to Trp enantiomers in the range of the concentration of [Cu(II)(Trp)(2)] from 5.0 x 10(-4) to 2.5 mM with a low limit of detection of 0.17 mu M (S/N = 3). The binding constant was calculated to be 2.97 x 10(3) M-1 for [Cu(II)(L-Trp)(2)] and 2.50 x 10(2) M-1 for [Cu(II)(L-Trp)(2)]. (C) 2013 Elsevier B.V. All rights reserved.