▎ 摘　　要

In this article, we described an approach for coupling of short DNAs with reduced graphene oxide and thus formation of transducer layer for biological sensors. We investigated the dependence of coupling ratio on the graphene oxide reduction level. We found optimal reduction parameters and showed successful conjugation of aptamers with reduced graphene oxide. We have revealed a trend to increase aptamer conjugation efficiency with a decrease of graphene oxide reduction rate. Finally, we made biosensor structures with pi-shaped reduction pattern and showed excellent sensitivity of the sensor during thrombin exposure. These results are important for the development of flexible low-cost biosensors of a new generation.